Description
Description: This Human Thymosin beta-4 (TMSB4X) ELISA Kit employs a two-site sandwich ELISA to quantitate TMSB4X in samples. An antibody specific for TMSB4X has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TMSB4X present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMSB4X is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMSB4X bound in the initial step. The color development is stopped and the intensity of the color is measured.